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Human Reproduction Update, Vol.1, No.1 pp.73-79, 1995
© © Oxford University Press

other

TEST-yolk media and sperm quality

R.S. Jeyendran1,2,3,1, V.K. Gunawardana1,4,1, D. Barisic1,5 and A.C. Wentz2

1Andrology Laboratory Services, Inc. 680 North Lake Shore Drive, Suite 1025, Chicago, IL 60611; 2Department of Obstetrics and Gynecology, Northwestern University Medical School Chicago, IL, USA 3Department of Physical Medicine and Rehabilitation, Northwestern University Medical School Chicago

To whom correspondence should be addressed at: 1To whom correspondence should be addressed at Andrology Laboratory Services

Media prepared with egg yolk and two buffers, TES and Tris, called TEST-yolk have been shown to have beneficial effects on the survivability, fertilizing capacity and storage potential of human spermatozoa. Egg yolk lipoproteins are the critical compounds for the beneficial effects, with a synergistic effect due to the TES-Tris buffer component TEST-yolk media have been used in the sperm penetration assay, the hemizona assay, sperm preparation for clinical in-vitro fertilization, artificial insemination with homologous spermatozoa, cryopreservation, sperm samples with a positive antisperm antibody test, and preparation for techniques requiring capacitated spermatozoa. Few harmful consequences of TEST-yolk have been reported, although controlled trials are required to evaluate therapeutic effects in the treatment of male infertility.

Key words: egg yolk / spermatozoa / TEST-yolk media


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