Skip Navigation


Human Reproduction Update Advance Access originally published online on November 29, 2004
Human Reproduction Update 2005 11(1):33-41; doi:10.1093/humupd/dmh050
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
11/1/33    most recent
dmh050v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wilton, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wilton, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction Update vol. 11 no. 1 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Preimplantation genetic diagnosis and chromosome analysis of blastomeres using comparative genomic hybridization

Leeanda Wilton

Genetic and Molecular Research, Melbourne IVF, 320 Victoria Parade, East Melbourne 3002, Victoria, Australia

Email: lwilton{at}mivf.com.au

Numerical chromosome errors are known to be common in early human embryos and probably make a significant contribution to early pregnancy loss and implantation failure in IVF patients. Over recent years fluorescent in situ hybridization (FISH) has been used to document embryonic aneuploidies. Many IVF laboratories perform preimplantation genetic diagnosis (PGD) with FISH to select embryos that are free from some aneuploidies in an attempt to improve implantation, pregnancy and live birth rates in particular categories of IVF patients. The usefulness of FISH is limited because only a few chromosomes can be detected simultaneously in a single biopsied cell. Complete karyotyping at the single cell level can now be achieved by comparative genomic hybridization (CGH). CGH enables not only enumeration of all chromosomes but gives a more complete picture of the entire length of each chromosome and has demonstrated that chromosomal breakages and partial aneuploidies exist in embryos. CGH has provided invaluable information about the extent of mosaicism and aneuploidy of all chromosomes in early human conceptuses. CGH has been applied to clinical PGD and has resulted in the birth of healthy babies from embryos whose full karyotype was determined in the preimplantation phase.

Key words: aneuploidy / comparative genomic hybridization / fluorescent in situ hybridization / preimplantation genetic diagnosis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
C. Le Caignec, C. Spits, K. Sermon, M. De Rycke, B. Thienpont, S. Debrock, C. Staessen, Y. Moreau, J.-P. Fryns, A. Van Steirteghem, et al.
Single-cell chromosomal imbalances detection by array CGH.
Nucleic Acids Res., January 1, 2006; 34(9): e68 - e68.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Gutierrez-Mateo, J. Benet, H. Starke, M. Oliver-Bonet, S. Munne, T. Liehr, and J. Navarro
Karyotyping of human oocytes by cenM-FISH, a new 24-colour centromere-specific technique
Hum. Reprod., December 1, 2005; 20(12): 3395 - 3401.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R. P. Dickey and R. Pyrzak
Preimplantation genetic screening will play a dominant role in selecting embryos for single embryo transfer
Hum. Reprod., October 1, 2005; 20(10): 2978 - 2978.
[Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.