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Human Reproduction Update Advance Access originally published online on April 7, 2005
Human Reproduction Update 2005 11(4):425-435; doi:10.1093/humupd/dmi009
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Possible applications of a non-contact 1.48 µm wavelength diode laser in assisted reproduction technologies

T. Ebner1, M. Moser and G. Tews

Women's General Hospital, IVF-Unit, Lederergasse 47, A-4020 Linz, Austria

1 To whom correspondence should be addressed. Email: thomas.ebner{at}gespag.at

Recently, one laser system has been introduced in IVF fulfilling all safety requirements, while achieving a high standard of reproducibility in terms of ablation diameter. This 1.48 µm wavelength indium-gallium-arsenic-phosphorus (InGaAsP) semiconductor laser offers a variety of laser applications to the embryologist. On the one hand, zona pellucida of oocytes or embryos can be manipulated in order to facilitate ICSI or biopsy and assist hatching, and on the other, spermatozoa may be paralysed or immobilized prior to usage. To conclude, the 1.48 µm diode laser provides a promising tool for the microdissection of subcellular targets. The diode laser stands out due to the rapidity, the simplicity and the safety of the procedure which is supported by healthy offspring after laser application.

Key words: assisted hatching / biopsy / diode laser / immobilization of spermatozoa / zona pellucida


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T. Mukaida, C. Oka, T. Goto, and K. Takahashi
Artificial shrinkage of blastocoeles using either a micro-needle or a laser pulse prior to the cooling steps of vitrification improves survival rate and pregnancy outcome of vitrified human blastocysts
Hum. Reprod., December 1, 2006; 21(12): 3246 - 3252.
[Abstract] [Full Text] [PDF]



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