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Human Reproduction Update, Vol.5, No.5 pp.553-560, 1999
© European Society of Human Reproduction and Embryology 1999; all rights reserved

Development of the human dispermic embryo

AH Sathananthan0,1, JJ Tarin2, L Gianaroli3, SC Ng4, V Dharmawardena0, MC Magli3, R Fernando0 and AO Trounson0

0 Institute of Reproduction and Development, Monash Medical Centre, Melbourne, Australia 2 Animal Biology, University of Valencia, Valencia, Spain 3 S.I.S.M.E.R. Srl, Bologna, Italy 4 Obstetrics and Gynecology, National University Hospital, Singapore 1 Corresponding author e-mail: henry.sathananthan@med.monashe.edu.au

Abstract

In a recent CD-ROM, we portrayed the microstructure of the pre-implantation human embryo (Sathananthan et al., 1999), which was a multimedia production with computer colour-enhanced electron micrographs of mainly monospermic embryos. This disk portrays light and electron micrographs of over 250 tripronuclear (3PN), dispermic, human embryos during pre-implantation development, viewed in thick and thin Araldite sections, as well as appearances of whole embryos flat embedded in Araldite blocks visualized with the light microscope. The 100 figures were computerized (IBM TIFF format), edited and labelled using Adobe Photoshop 5. Some of the figures were coloured on computer. The early development of 3PN embryos overtly resembles that of normal embryos but there are important differences in their microstructure which are portrayed in this presentation. This is a multicentric study involving researchers from four IVF centres.


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[Abstract] [Full Text] [PDF]



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