Human Reproduction Update, Vol.9, No.6 pp.557-582, 2003
© European Society of Human Reproduction and Embryology 2003; all rights reserved
Gamete/Embryo Physiology |
Chemically defined media and the culture of mammalian preimplantation embryos: historical perspective and current issues
1 Reproductive Science Center, Lexington, MA and Department of Obstetrics and Gynecology, University of Massachusetts Medical School, Worcester, MA and 2 Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
To whom correspondence should be addressed at: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. e-mail: john_biggers{at}hms.harvard.edu
Abstract
Considerable advances in media development for the culture of preimplantation mammalian embryos have been made since mouse embryos were first cultured and successfully transferred to foster mothers. The purpose of this review is to detail the history of the development of chemically defined media for the culture of preimplantation embryos. Two approaches have been used to determine the composition of chemically defined media: the ‘back-to-nature’ approach and ‘let the embryo choose’ or empirical optimization approach. Recent developments, including the supplementation of media with amino acids and the use of sequential media for the extended culture of preimplantation embryos, are critically assessed. Importantly, it is recognized that even the best media currently used are not optimal and inevitably cause imbalances and stress to the embryos. Consequently, preimplantation embryos must adapt to the culture environment in order to survive. The adaptations to stress that occur when embryos are placed in a chemically defined environment are reviewed. The implications of these various stresses on the patterns of gene expression in the early embryo and their potential long-term effects are also emphasized. The scientific and ethical issues raised by the commercialization of human embryo culture media are briefly addressed.
Key words: amino acids / culture media / extended culture / KSOM / sequential media
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