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Human Reproduction Update, Vol.9, No.6 pp.583-605, 2003
© European Society of Human Reproduction and Embryology 2003; all rights reserved

Embryology

Terminology associated with vitrification and other cryopreservation procedures for oocytes and embryos

J.M. Shaw1 and G.M. Jones1

1 Monash Institute of Reproduction and Development, Monash University, Clayton, Victoria 3168, Australia

To whom correspondence should be addressed at: e-mail: Jill.Shaw{at}Med.Monash.edu.au

Abstract

Over the past 30 years many cryopreservation procedures have been applied to oocytes, embryos, sperm, ovarian and testicular tissue. Over this time many, often specialized, terms have been developed for all aspects of these procedures. This can make it difficult for readers who are not familiar with the terminology or protocols to compare and evaluate different procedures. This paper describes the main cryopreservation procedures, the terminology associated with them, and briefly explains the underlying physical, chemical and biological processes. The aim is to help readers understand and interpret other papers on slow cooling, rapid cooling, ultrarapid cooling and vitrification.

Key words: cooling / cryopreservation / embryos / oocytes / vitrification


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