Human Reproduction Update Advance Access published online on October 12, 2009
Human Reproduction Update, doi:10.1093/humupd/dmp045
Cell volume regulation in oocytes and early embryos: connecting physiology to successful culture media
Ottawa Hospital Research Institute, and Departments of Obstetrics and Gynecology (Division of Reproductive Medicine), and Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada K1Y 4E9
To whom correspondence should be addressed at: 1 Correspondence address. E-mail: jmbaltz{at}ohri.ca
BACKGROUND: Preimplantation embryos are particularly susceptible to in vitro developmental blocks. These could be alleviated by lowering culture medium osmolarity. Because mammalian cells regulate their volumes by adjusting intracellular osmotic pressure, cell volume regulation could be critical to early embryos.
METHODS: We reviewed the literature on cell volume regulation in preimplantation embryos and the effects of increased osmolarity on embryo development, focusing also on the relation with improvements in embryo culture media.
RESULTS: Embryos failed to develop from fertilized oocytes when osmolarity is increased. This could be alleviated by decreasing osmolarity or including certain compounds such as certain amino acids. Early preimplantation mouse embryos require intracellular accumulation of glycine to provide osmotic support and thus control cell volume. The glycine-specific transporter, GLYT1, mediates osmoregulated glycine accumulation in mouse embryos and likely in human embryos. GLYT1 is activated during meiotic maturation starting at ovulation. Prior to this, oocyte size is not independently controlled but instead is determined by strong adhesion between the oocyte plasma membrane and the inner surface of the zona pellucida.
CONCLUSIONS: Early preimplantation embryos are particularly sensitive to increased osmolarity, and require the importation of glycine to regulate their cell volumes using a mechanism unique to early embryos. Cell volume regulation first appears when ovulation is triggered, oocyte zona pellucida adhesion is released, and glycine transport is activated. The requirement for supporting these physiological functions in oocytes and embryos should be taken into account when developing and improving systems for in vitro oocyte maturation and embryo culture.
Key words: cell volume / glycine / oocyte / osmolyte / preimplantation
Received on July 24, 2009; revised September 10, 2009; accepted on September 11, 2009