Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Harkness, L.
Right arrow Articles by Baird, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harkness, L.
Right arrow Articles by Baird, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction Update, Vol.3, No.1 pp.59-78, 1997
© European Society of Human Reproduction and Embryology 1997; all rights reserved

Morphological and molecular characteristics of living human fetuses between Carnegi stages 7 and 23: localization of inhibin mRNA {alpha} and ßa subunits by in-situ hybridization

LM Harkness0 and DT Baird

Department of Obstetrics and Gynaecology, University of Edinburgh, 37 Chalmers Street, Edinburgh EH3 9EW, UK 0 Corresponding author

Abstract

Localization of the mRNA {alpha} and ßa subunits of inhibin has previously been reported in the human gonads during the second trimester. Adrenal inhibin has also been reported in the second trimester for the {alpha} ßa and ßb subunits. Investigations showing localization by in-situ hybridization during the first trimester have not been reported. The results have shown hybridization of the {alpha} and ßa subunits, throughout the period of development studied, in a variety of tissues including the dorsal and thoracic aortas and pericardium stages 13-22 (ßa subunit); liver stages 19-21 (ßa) and stages 21-22 ({alpha}); mesonephros stages 21 and 22 (ßa); gonad stages ({alpha} and ßa); adrenal stages 19-22 ({alpha}); surface ectoderm stages 16-22 (ßa); mesenchyme stages 16-22 (ßa); amnion stages 13-16 (ßa); yolk sac stage 12 ({alpha} and ßa); cartilage stages 19-22 (ßa); and nasal proliferation stages 21 and 22 (ßa). When compared with distribution of the protein subunits it was noted that more immunostaining activity was found, suggesting that probes were not sufficiently sensitive enough to detect all levels of mRNA expressed. It can be surmised, therefore, that the lack of visual hybridization of the mRNA cannot preclude the possibility that it is not being translated within the tissue even though hybridization was not apparent.

Keywords: activin/embryo/inhibin/in-situ hybridization


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
C. Welt, Y. Sidis, H. Keutmann, and A. Schneyer
Activins, Inhibins, and Follistatins: From Endocrinology to Signaling. A Paradigm for the New Millennium
Experimental Biology and Medicine, October 1, 2002; 227(9): 724 - 752.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.