Human Reproduction Update, Vol.3, No.2 pp.117-123, 1997
© European Society of Human Reproduction and Embryology 1997; all rights reserved
Human endometriosis-derived permanent cell line (FbEM-1): establishment and characterization
0 Service de Gynecologie-Obstetrique, Hopital Beaujon, Clichy, France 1 Laboratoire d'Anatomie Pathologique, Institut Curie, Paris, France 2 Service de Gynecologie-Obstetrique, CHU-Clermont Ferrand and 3 INSERM U90, Hopital Necker, Rue de Sevres, 75015-Paris, France z Corresponding author
Abstract
A human epithelial-like cell line derived from peritoneal implants from a patient with gonadotrophin-releasing hormone (GnRH) agonist-resistant endometriosis graded as stage IVd according to the American Fertility Society classification was established in vitro. This cell line, designated FbEM-1, exhibited an epithelial-like morphology, grew in suspension and was immunoreactive for cytokeratins 8, 18, 19, vimentin and human leukocyte class I antigens. The cultured cells were negative for various haematopoietic cell markers, including the lymphoid cell antigens CD3, CD20 and CD45, von Willebrandt factor, carcinoembryonic antigen and the carcinoma antigen-125 (CA-125). In addition, the FbEM-1 cells were found to be moderately positive for periodic acid Schiff's (PAS) solution but were negative for 
-naphthyl acetate esterase, peroxidase and chloroacetate esterase activities. Using specific antibodies against the progesterone, androgen and oestrogen receptors, 40% and 5-10% of the cells immunostained for progesterone and androgen receptors respectively, while oestrogen receptors were not detected. On cytogenetic analysis using R-banding, these cells showed numerous chromosomal aberrations, including loss of one chromosome X, 4q+, 5q+, trisomy 7, 8 and 10 and tetrasomy of chromosomes 17, 18, 19 and 20. These data show that the continuously growing FbEM-1 cell line established from endometriotic implants may be useful in achieving better understanding of the histogenesis of endometriosis.
Keywords: cell line/endometriosis/karyotype/monoclonal antibodies
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W.-P. Hu, S. K. Tay, and Y. Zhao Endometriosis-Specific Genes Identified by Real-Time Reverse Transcription-Polymerase Chain Reaction Expression Profiling of Endometriosis Versus Autologous Uterine Endometrium J. Clin. Endocrinol. Metab., January 1, 2006; 91(1): 228 - 238. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gogusev, J.B. de Joliniere, L. Telvi, M. Doussau, S. du Manoir, A. Stojkoski, and M. Levardon Genetic abnormalities detected by comparative genomic hybridization in a human endometriosis-derived cell line Mol. Hum. Reprod., September 1, 2000; 6(9): 821 - 827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gogusev, J. B. de Joliniere, L. Telvi, M. Doussau, A. Stojkoski, and M. Levradon Cellular and Genetic Constitution of Human Endometriosis Tissues Reproductive Sciences, March 1, 2000; 7(2): 79 - 87. [Abstract] [PDF]
|
|