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Human Reproduction Update Advance Access published online on April 7, 2005

Human Reproduction Update, doi:10.1093/humupd/dmi009
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received November 24, 2004
Revised January 26, 2005
Accepted January 27, 2005

Review

Possible applications of a non-contact 1.48 µm wavelength diode laser in assisted reproduction technologies

T. Ebner 1*, M. Moser 1, and G. Tews 1

1 Women's General Hospital, IVF-Unit, Lederergasse 47, A-4020 Linz, Austria


   Abstract

Recently, one laser system has been introduced in IVF fulfilling all safety requirements, while achieving a high standard of reproducibility in terms of ablation diameter. This 1.48 µm wavelength indium-gallium-arsenic-phosphorus (InGaAsP) semiconductor laser offers a variety of laser applications to the embryologist. On the one hand, zona pellucida of oocytes or embryos can be manipulated in order to facilitate ICSI or biopsy and assist hatching, and on the other, spermatozoa may be paralysed or immobilized prior to usage. To conclude, the 1.48 µm diode laser provides a promising tool for the microdissection of subcellular targets. The diode laser stands out due to the rapidity, the simplicity and the safety of the procedure which is supported by healthy offspring after laser application.

Keywords: assisted hatching; biopsy; diode laser; immobilization of spermatozoa; zona pellucida.
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